ViroKey SQ FLEX SARS-CoV-2 Genotyping Assay (RUO)

Detection for mutation, through whole genome sequencing of SARS-CoV-2

DESCRIPTION

The ViroKey SQ FLEX SARS-CoV-2 Genotyping Assay is a next-generation sequencing-based test intended for the detection of genomic mutations of the SARS-CoV-2 RNA genome from nasopharyngeal or oropharyngeal swab samples to support molecular epidemiology of SARS-CoV-2 research.

The  ViroKey SQ FLEX SARS-CoV-2 Genotyping Assay is used in conjunction with the Sentosa SX Virus Total Nucleic Acid Plus (4x24) as well as the Sentosa SQ Virus Testing Solutions on the Sentosa SX101, Sentosa ST401 and Sentosa SQ301 instruments.

FEATURES AND BENEFITS

High Performance and Robustness:

  • > 99% coverage of the SARS-CoV-2 genome
  • ≥ 200x depth of coverage

Providing Laboratory Efficiency and Effectiveness:

  • Able to extract up to 15 samples (plus controls) per run
  • Reliable and reproducible variant calling
  • Automated workflow, from samples to results in 2 days
  • Minimal hands-on time of < 2 hours
  • Automatic bioinfomatics analysis and report generation (including lineage information) with Sentosa SQ Reporter

WORKFLOW

SPECIFICATIONS

Parameters Specification
Sample Types

Nasopharyngeal and Oropharyngeal Swabs in UVT or UTM 

Sample Input

730 μL 

Sample Throughput

Up to 15 samples per run 

Controls

System Control, Extraction Control, Positive Control (Optional) 

Coverage

≥ 200X 

Limit of Detection (LoD)

1000 GE/mL 

Hands-on Time

< 2 hours 

Turnaround Time

~2 days (from sample to results) 

PRODUCT FLYERS

ORDERING INFORMATION

ProductPack SizeItem Number
Sentosa SQ Virus Testing Solutions (CE-IVD) 4x24 300778
Sentosa SQ Virus Testing Solutions (RUO) 4x24 300775
Sentosa SQ Virus Testing Solutions (US-IVD) 4x24 300732
Sentosa SX Virus Total Nucleic Acid Plus Kit 4x24 300395
Sentosa SX Virus Total Nucleic Acid Plus Kit (CE-IVD) 4x24 300154
Sentosa SX Virus Total Nucleic Acid Plus Kit (RUO) 4x24 300155
ViroKey SQ FLEX Library Prep Reagents (4x16) (RUO) 4x16 300823
ViroKey SQ FLEX SARS-CoV-2 Positive Control Kit (8) (RUO) 8 301092
ViroKey SQ FLEX SARS-CoV-2 Primer Set (96) (RUO) 96 301091