ViroKey SQ FLEX Genotyping Assay

Flexibility for virology, combine up to 4 assays (HIV-1, HCV, HBV, CMV) in one NGS run


The ViroKey® SQ FLEX Genotyping Assay (4x16) is a next generation sequencing-based test intended for the detection of genomic mutations of the Human Immunodeficiency virus (HIV-1), Hepatitis C virus (HCV), Hepatitis B virus (HBV) and Cytomegalovirus (CMV) that confer resistance to specific types of antiviral drugs from human plasma / serum. Up to 4 tests can be performed in one NGS run.

The  ViroKey SQ FLEX Genotyping Assay (4x16) is used in conjunction with the Sentosa® SX Virus Total Nucleic Acid Plus (4x24) as well as the Sentosa SQ 318 Chip Kits, Sentosa ST Template Kits and the Sentosa SQ Sequencing Kits on the Sentosa SX101, Sentosa ST401 and Sentosa SQ301 instruments.


TargetTarget GenesTarget Genotypes and Subtypes

Reverse Transcriptase, Protease and Integrase regions of pol gene

Group M subtypes


 NS3, NS5A, NS5B

Genotyping: Genotypes 1 to 6, subtypes 1a/1b2

 Detecting mutations:  Subtypes 1a/1b, Genotype 3


Reverse Transcriptase, Surface gene, Basal Core Promoter, PreCore and Core regions.



UL54, UL97, UL56, UL51, UL27, UL 89



Supporting Clinical Research:

  • Up-to-date databases: Stanford, ANRS, REGA, EASL and AASLD Guidelines, DAA IFU
  • Provides drug resistance intepretation report
  • Solution for low volume testing

Assay Selection Flexibility:

  • Freedom to run up to 4 assays in one run
  • Genotype and resistance information in a single test
  • Easy menu expansion to add new assays

Laboratory Efficiency and Effectiveness

  • Able to extract up to 16 samples (including controls) per run
  • Seamless sample traceability and network connectivity
  • Automated workflow, from sample-to-results in 2.5 days and less than 4 hours of hands-on time
  • Automated data analysis and result reporting



Sample Input

730 μl Plasma for HIV, HCV, HBV, CMV
730 μl Serum for HCV, HBV

Sample Throughput Up to 16 samples/run (assay dependent)
Analytical Sensitivity 1,000 copies/mL for HIV-1
1,000 IU/mL for HCV, HBV, CMV


ViroKey & Sentosa FLEX Flyer RUO EN



ProductPack SizeItem Number
Sentosa SX Virus Total Nucleic Acid Plus Kit (4x24) RUO 4x24 300155
ViroKey SQ CMV Positive Control Kit (8) RUO 8 301054
ViroKey SQ FLEX CMV Primer Set (96) RUO 96 300822
ViroKey SQ FLEX HBV Primer Set (96) RUO 96 300821
ViroKey SQ FLEX HCV Primer Set (96) RUO 96 300820
ViroKey SQ FLEX HIV-1 Primer Set (96) RUO 96 300819
ViroKey SQ FLEX Library Prep Reagents (4x16) RUO 4x16 300823
ViroKey SQ HBV Positive Control Kit (8) RUO 8 300761
ViroKey SQ HCV Positive Control Kit (8) RUO 8 300824
ViroKey SQ HIV-1 Positive Control Kit (8) RUO 8 300683