Sentosa SQ HCV Genotyping Assay v2.0 (USA)

Sequence and genotype Hepatitis C Virus samples


The Sentosa® SQ HCV Genotyping Assay v2.0 uses next-generation sequencing (NGS)-technology to deliver accurate detection of Hepatitis C Virus genotypes 1-6 and their associated subtypes as well as identification of resistance-associated substitutions (RASs).

Compared with traditional methods for HCV genotyping such as line-probe assays (LiPA), this NGS assay eliminates genotyping errors while simultaneously delivering information about RASs found in the sample

For Research Use Only. Not for use in diagnostic procedures.  


Target GenesNumber of AmpliconsTarget Genotypes and Subtypes
NS5B 1 1a, 1b, 2, 3, 4, 5, 6
NS3 1 1a, 1b
NS5A 1 1a, 1b
System Control 1 NA
Total 4  


  • Delivers genotyping & RAS identification accuracy in a single, efficient workflow
  • Reliable, automated solution – full workflow automation combined with built-in controls give you confidence in your results
  • Speeds time to next steps – simultaneously analyze genotyping and RAS-calling with minimal hands-ontime using our automated solution
  • Expert assay design – multiplexed sequencing design targets key regions indicated in AASLD guidelines including genotypes 1 through 6 as well as 70 subtypes enabling discovery of potential new relationships between subtypes and novel therapeutics



Sample Input 730 μL serum or plasma
Sample Throughput 15 patient samples and 1 system control
Mean Coverage / Target ≥ 200 X for genotyping, ≥ 500 X for variant calling
Analytical Sensitivity ≥ 1,000 HCV IU/mL for genotypes 1a, 1b, 2, 3, and 4
≥ 2,000 HCV IU/mL for genotypes 5 and 6
Analytical Specificity No cross-reactivity with HAV, HBV, HIV, CMV, EBV, VZV, HHV1/2, Dengue virus or human genomic DNA
Reproducibility 99.20%


Sentosa SQ HCV Genotyping Assay Flyer RUO EN



ProductPack SizeItem Number
Sentosa SQ 318 Chip Kit (8) RUO 8 runs 300301
Sentosa SQ HCV Genotyping Reagents v2.0 (4x16) RUO 4x16 300393
Sentosa SQ Sequencing Reagents (8) RUO 8 300310
Sentosa SQ Sequencing Solutions (8) RUO 8 300314
Sentosa SQ Sequencing Supplies (8) RUO 8 300304
Sentosa SQ Virus Testing Solutions (4x24) RUO 4x24 300775
Sentosa SQ Virus Testing Solutions (RUO) 4x24 300775
Sentosa ST Template Beads (8) RUO 8 300320
Sentosa ST Template Reagents (8) RUO 8 300317
Sentosa ST Template Solutions (8) RUO 8 300323
Sentosa ST Template Supplies (8) RUO 8 300326
Sentosa SQ HCV Positive Control v2.0 (8) RUO 8 301056
Sentosa SQ HCV Positive Control v2.0 (96) RUO 96 300757
Sentosa SX Virus Total Nucleic Acid Plus II Kit (4x16) RUO 4x16 300352