Sentosa SQ HCV Genotyping Assay v2.0 (USA)
Sequence and genotype Hepatitis C Virus samples
The Sentosa® SQ HCV Genotyping Assay v2.0 uses next-generation sequencing (NGS)-technology to deliver accurate detection of Hepatitis C Virus genotypes 1-6 and their associated subtypes as well as identification of resistance-associated substitutions (RASs).
Compared with traditional methods for HCV genotyping such as line-probe assays (LiPA), this NGS assay eliminates genotyping errors while simultaneously delivering information about RASs found in the sample
For Research Use Only. Not for use in diagnostic procedures.
| Target Genes | Number of Amplicons | Target Genotypes and Subtypes |
|---|---|---|
| NS5B | 1 | 1a, 1b, 2, 3, 4, 5, 6 |
| NS3 | 1 | 1a, 1b |
| NS5A | 1 | 1a, 1b |
| System Control | 1 | NA |
| Total | 4 |
- Delivers genotyping & RAS identification accuracy in a single, efficient workflow
- Reliable, automated solution – full workflow automation combined with built-in controls give you confidence in your results
- Speeds time to next steps – simultaneously analyze genotyping and RAS-calling with minimal hands-ontime using our automated solution
- Expert assay design – multiplexed sequencing design targets key regions indicated in AASLD guidelines including genotypes 1 through 6 as well as 70 subtypes enabling discovery of potential new relationships between subtypes and novel therapeutics
| Attribute | Value |
|---|---|
| Sample Input | 730 μL serum or plasma |
| Sample Throughput | 15 patient samples and 1 system control |
| Mean Coverage / Target | ≥ 200 X for genotyping, ≥ 500 X for variant calling |
| Analytical Sensitivity | ≥ 1,000 HCV IU/mL for genotypes 1a, 1b, 2, 3, and 4 ≥ 2,000 HCV IU/mL for genotypes 5 and 6 |
| Analytical Specificity | No cross-reactivity with HAV, HBV, HIV, CMV, EBV, VZV, HHV1/2, Dengue virus or human genomic DNA |
| Reproducibility | 99.20% |
PRODUCT FLYERS
| Sentosa SQ HCV Genotyping Assay Flyer | RUO | EN |
| Product | Pack Size | Item Number |
|---|---|---|
| Sentosa SQ 318 Chip Kit (8) RUO | 8 runs | 300301 |
| Sentosa SQ HCV Genotyping Reagents v2.0 (4x16) RUO | 4x16 | 300393 |
| Sentosa SQ Sequencing Reagents (8) RUO | 8 | 300310 |
| Sentosa SQ Sequencing Solutions (8) RUO | 8 | 300314 |
| Sentosa SQ Sequencing Supplies (8) RUO | 8 | 300304 |
| Sentosa SQ Virus Testing Solutions (4x24) RUO | 4x24 | 300775 |
| Sentosa SQ Virus Testing Solutions (RUO) | 4x24 | 300775 |
| Sentosa ST Template Beads (8) RUO | 8 | 300320 |
| Sentosa ST Template Reagents (8) RUO | 8 | 300317 |
| Sentosa ST Template Solutions (8) RUO | 8 | 300323 |
| Sentosa ST Template Supplies (8) RUO | 8 | 300326 |
| Sentosa SQ HCV Positive Control v2.0 (8) RUO | 8 | 301056 |
| Sentosa SQ HCV Positive Control v2.0 (96) RUO | 96 | 300757 |
| Sentosa SX Virus Total Nucleic Acid Plus II Kit (4x16) RUO | 4x16 | 300352 |
