Sentosa SA ZIKV-DENV-CHIKV RT-PCR Test
Determination of Tropical Infections
Detect and differentiate 3 different mosquito-bourne viruses in a single sample with the Sentosa® SA
ZIKV-DENV-CHIKV RT-PCR Test. Our real-time PCR assay enables you to identify the presence of Zika, Dengue,
and Chikungunya virus in under 3 hours with only 250 uL of sample input required.
Simultaneous Detection and Di erentiation of Tropical Viruses
As presented at the 2016 American Society of Tropical Medicine and Hygeine, co-infection of Dengue,
Chikungunya and Zika may occur with a single Aedes mosquito bite. These three viruses present similar
symptoms, but yield different outcomes, and furthermore, the presence of multiple viruses may produce
even greater clinical impact. In order to further understand these viral infections, more clinical research is
necessary. The Sentosa® SA ZIKV-DENV-CHIKV RT-PCR Assay enables a simple, single workflow to detect and
differentiate these viruses from a single sample.
RUO: For Research Use Only.
WORKFLOW
- Single multiplex test enables detection of Zika, Dengue, and Chikungunya including 82 strains of Zika and all 4 serotypes of Dengue
- HIgh analytical performance with no cross-reactivity
- Low detection limit enables detection of samples with low viral load, only 250 uL of sample input needed
- Automated solution delivers results in about 3 hours
| Attribute | Zika | Dengue | Chikungunya |
|---|---|---|---|
| Target Gene | ZIKV MR-766 and ZIKV PRAVBC59 | 3'UTR | E1 gene |
| Analytical Sensitivity (95% CI) | ZIKV MR-766: 6 x 103 copies/mL ZIKV PRAVBC59: 3 x 103 copies/mL |
9 pfu/ml;7.5 copies/uL |
31.0 pfu/mL; 9.0 copies/uL |
| Analytical Reactivity | 82 strains tested & detected | 4 strains tested & detected | 1 strain tested & detected |
| Analytical Specificity | No cross reactivity with 44 pathogens tested | No cross reactivity with 13 pathogens tested | No cross reactivity with 16 pathogens tested |
| Sample Type(s) | EDTA plasma, serum or urine (collected alongside a patient-matched EDTA plasma and / or serum specimen) | EDTA plasma and serum | EDTA plasma and serum |
| Test combination | One sample required to run all 3 tests | ||
| Controls | Postivite control (PC), Negative control (NC), & Extraction Control (EC) | ||
| Automation | Lysis, extraction & PCR setup | ||
| Product | Pack Size | Item Number |
|---|---|---|
| Sentosa SX Virus Total Nucleic Acid Kit v2.0 (4x24) US-IVD | 4x24 | 300353 |
For availability in your country, please contact your Vela Diagnostics sales representative.
