Using an innovative rotary design, the Rotor-Gene® Q MDx 5plex HRM Platform allows for unmatched thermal performance in real-time PCR cycling. The use of a rotating disc ensures that every single tube is spun in a chamber of uniform temperature, using air as the conducting element. This allows for rapid thermal cycling and precise results in all tubes.
For detection, a set of 6 uncoupled excitation and emission filters are used in conjunction with 6 dedicated LEDs to maximize the detection capability. Each tube is illuminated by the excitation LED and the fluorescent signal transmitted to the PMT detector via a uniform optical path length. This eliminates well-to-well optical variation due to edge effect which block-based cycler had experienced. It also allows for fluorescent detection without the need of a reference dye.
With thermal and optical uniformity, the Rotor-Gene® Q MDx 5plex HRM Platform offers unparalleled quantitative and genotyping analysis for PCR applications.
With 5 channels offering detection from the green to infrared wavelengths, the Rotor-Gene® Q MDx platform provides wide optics for most fluorophores available in the market. The decoupled filter sets create flexibility for customized excitation/detection wavelength combinations, making the platform compatible with most fluorophores.
|Channel||Excitation (nm)||Detection (nm)||Examples of fluorophores detected|
|Green||470±10||510±5||FAM, YYBR Green I, Fluorescein, EvaGreen, Alexa Fluor 488|
|Yellow||530±5||557±5||JOE, VIC, HEX, TET, MAX, CAL Fluor Gold 540, Yakima Yellow|
|Orange||585±5||610±5||ROX, CAL Fluor Red 610, Cy 3.5, Texas Red, Alexa Fluor 568|
|Red||625±5||660±10||Cy5, Quasar 670, LightCycler Red640, Alexa Fluor 663|
|Crimson||680±5||712 high pass||Quasar 705, LightCycler Red 705, Alexa Fluor 680|
|High Resolution Melt (HRM)||460±20||510±5||SYBR Green I, SYTO 9, LC Green, LC Green Plus+, EvaGreen|
|Product||Pack Size||Item Number|
|Sentosa SX qPCR Starter Kit||1||400107|
|Rotor-Gene® Q MDx||1||400046|