Rotor-Gene® Q MDx
5plex HRM platform
Using an innovative rotary design, the Rotor-Gene® Q MDx 5plex HRM Platform allows for unmatched thermal performance in real-time PCR cycling. The use of a rotating disc ensures that every single tube is spun in a chamber of uniform temperature, using air as the conducting element. This allows for rapid thermal cycling and precise results in all tubes.
For detection, a set of 6 uncoupled excitation and emission filters are used in conjunction with 6 dedicated LEDs to maximize the detection capability. Each tube is illuminated by the excitation LED and the fluorescent signal transmitted to the PMT detector via a uniform optical path length. This eliminates well-to-well optical variation due to edge effect which block-based cycler had experienced. It also allows for fluorescent detection without the need of a reference dye.
With thermal and optical uniformity, the Rotor-Gene® Q MDx 5plex HRM Platform offers unparalleled quantitative and genotyping analysis for PCR applications.
With 5 channels offering detection from the green to infrared wavelengths, the Rotor-Gene® Q MDx platform provides wide optics for most fluorophores available in the market. The decoupled filter sets create flexibility for customized excitation/detection wavelength combinations, making the platform compatible with most fluorophores.
| Channel | Excitation (nm) | Detection (nm) | Examples of fluorophores detected |
|---|---|---|---|
| Green | 470±10 | 510±5 | FAM, YYBR Green I, Fluorescein, EvaGreen, Alexa Fluor 488 |
| Yellow | 530±5 | 557±5 | JOE, VIC, HEX, TET, MAX, CAL Fluor Gold 540, Yakima Yellow |
| Orange | 585±5 | 610±5 | ROX, CAL Fluor Red 610, Cy 3.5, Texas Red, Alexa Fluor 568 |
| Red | 625±5 | 660±10 | Cy5, Quasar 670, LightCycler Red640, Alexa Fluor 663 |
| Crimson | 680±5 | 712 high pass | Quasar 705, LightCycler Red 705, Alexa Fluor 680 |
| High Resolution Melt (HRM) | 460±20 | 510±5 | SYBR Green I, SYTO 9, LC Green, LC Green Plus+, EvaGreen |
- Works in combination with Sentosa SX101 for upstream sample extraction and PCR set-up
- Thermal non-uniformity of ±0.02°C
- 5 sets of decoupled excitation/emission filters supporting a wide variety of dyes
- Optical uniformity allows PCR without the need of reference dye
- Rapid thermal heating and cooling using air as conducting element
- Optically stable LEDs with life time guarantee
- No optical calibration required during installation
- “Spin down” of tubes is not required
- Optical Temperature Verification (OTV) kit available for simple thermal verification for performance without the need for contacting a service technician
- Easy to clean, low maintenance & service requirement
- Small footprint (W37;H27.5;D42 cm) (W14.6;H10.8;D16.5 in)
| Product | Pack Size | Item Number |
|---|---|---|
| Rotor-Disc 72 Starter Kit (115V) and Accessories | 1 | 400047 |
| Rotor-Gene Q | 1 | 400046 |
