Sentosa SQ Thyroid Cancer Panel is a ready-to-use kit for automated PCR-based next generation sequencing library preparation using the Sentosa® SX101 instrument for the detection of mutations associated with thyroid cancer. This kit contains four sets of reagents and solutions; each set allows for the preparation, purification and normalization of eight libraries per run. In addition, Sentosa® SQ Thyroid Cancer Panel contains Thyroid Cancer SC (Thyroid Cancer System Control), which functions as an extraction control, a positive control and a no template control. The primer mix provided in this kit is designed for the specific amplification of target genes containing potential mutations as well as the system control fragment.
The Sentosa SQ Thyroid Cancer Panel is a next generation sequencing-based test that simultaneously detects hot spot mutations in 10 genes from formalin-fixed paraffin-embedded (FFPE) samples for research use only. This panel is intended to be used on the Sentosa® SX101 with the Sentosa SX FFPE II gDNA Kit in conjunction with the Sentosa ST401 and Sentosa SQ301 instruments.
Target Genes | Number of Amplicons | Number of Target Mutations | Amplicon Locations in Exon(s) |
---|---|---|---|
BRAF | 2 | 15 | 11, 15 |
CTNNB1 | 1 | 5 | 2 |
EGFR | 4 | 10 | 18, 19, 20, 21 |
FGFR3 | 3 | 9 | 7, 9, 14 |
KRAS | 3 | 22 | 2, 3, 4 |
NRAS | 3 | 19 | 2, 3, 4 |
PTEN | 3 | 3 | 5, 7 |
PIK3CA | 2 | 14 | 11, 22 |
RET1 | 1 | 1 | 16 |
TP53 | 3 | 7 | 4, 6, 7 |
Total | 25 | 105 |
Supporting Research Use
Providing Laboratory Efficiency
Attribute | Value |
---|---|
Sample Input | 10 μm section of FFPE tissue (on glass slide or scroll) |
Target Amplicons | 25 targets and 1 control |
Target Mutations | 105 |
Min. Coverage / Target Mutation | > 1,000 x |
Analytical Sensitivity | ≥ 5 ng DNA input; 5% variant frequency for mutation detection |
Clinical Sensitivity | 100% (95% CI: 95.07% to 100%) |
Clinical Specificity | 100% (95% CI: 95.42% to 100%) |
Reproducibility | ≥ 99.89% (95% CI: 99.82% to 99.93%) |
Product | Pack Size | Item Number |
---|---|---|
Sentosa SQ 318 Chip Kit (8) RUO | 8 runs | 300301 |
Sentosa SQ Oncology Solutions (4x8) RUO | 4x8 | 300224 |
Sentosa SQ Sequencing Reagents (8) RUO | 8 | 300310 |
Sentosa SQ Sequencing Solutions (8) RUO | 8 | 300314 |
Sentosa SQ Sequencing Supplies (8) RUO | 8 | 300304 |
Sentosa SQ Thyroid Cancer Reagents (4x8) RUO | 4x8 | 300382 |
Sentosa ST Template Beads (8) RUO | 8 | 300320 |
Sentosa ST Template Reagents (8) RUO | 8 | 300317 |
Sentosa ST Template Solutions (8) RUO | 8 | 300323 |
Sentosa ST Template Supplies (8) RUO | 8 | 300326 |
Sentosa SX FFPE II gDNA Kit (4x8) RUO | 4x8 | 300231 |
For availability in your country, please contact your Vela Diagnostics sales representative. Availability is subject to country's regulatory approval.