Sentosa® SQ OncoKey Select

Detect sequence variants in pan-cancer samples

INTENDED USE

The Sentosa SQ OncoKey Select Panel is a comprehensive next-generation sequencing (NGS)-based assay that simultaneously detects hotspot mutations in formalin-fixed paraffin-embedded (FFPE) samples.

This pan-cancer panel covers 80 genes and more than 500 mutations. Designed by scientists and clinicians, the Sentosa  SQ OncoKey Select Panel covers genes that are frequently mutated in human cancers and with demonstrated relevance for various ongoing clinical trials.

The Sentosa SQ OncoKey Select Panel is intended to be used on the Sentosa SX101 instrument in conjunction with the Sentosa SX FFPE II gDNA Kit and Sentosa SQ301 and Sentosa ST401 instruments. Up to 15 samples can be automatically processed and analysed within 2.5 days (including overnight data analysis). The Sentosa SQ OncoKey Select Panel is for research use only (RUO) and not for use in diagnostic procedures.

TARGET GENES

AKT1

ERBB2

JAK2

PTEN

AKT2

ERBB3

KDR

RAC1

AKT3

ERBB4

KEAP1

RB1

ALK

ESR1

KIT

RET

APC

EZH2

KMT2C

RHOA

AR

FAT1

KMT2D

ROS1

ARAF

FBXW7

KRAS

SF3B1

ARID1A

FGFR1

MAP2K1

SMAD4

BAP1

FGFR2

MAP2K2

SMARCB1

BRAF

FGFR3

MAP3K1

SMO

BRCA1

FOXL2

MET

SRC

BRCA2

GATA3

MLH1

STK11

CDH1

GNA11

MTOR

TP53

CDK4

GNAQ

NF1

TSC1

CDKN2A

GNAS

NFE2L2

TSC2

CSF1R

H3F3A

NOTCH1

U2AF1

CTCF

HIST1H3B

NRAS

VHL

CTNNB1

HNF1A

PDGFRA

 

DDR2

HRAS

PIK3CA

 

DICER1

IDH1

PIK3R1

 

EGFR

IDH2

POLE

 

FEATURES AND BENEFITS

  • Analyse up to 80 genes in one run
  • Detect 10% variant frequency for mutation
  • Sample input: only 30 ng of FFPE DNA required
  • Get accurate interpretation and reporting with Vela Genomics Service and TheraKey Solution
  • From sample to result in 2.5 days
  • Pay per gene: pay only for your selected genes of interest
  • Combine different solid tumor types in one run
  • Most relevant genes for enhanced tumor testing in one panel
  • Seamless sample traceability and IT connectivity
  • Four hours of hands on time

WORKFLOW

SPECIFICATIONS (AUTOMATED WORKFLOW)

Attribute Sentosa SQ OncoKey Select Panel
Regulatory Status RUO
Targeted Cancer Pan-Cancer
Number of Genes 80 (incl 3 whole genomes)
Number of Amplicons 382
Target Mutations 577
Sample Type FFPE
Sample Input 30 ng
Sample Throughput Up to 15 samples + 1 system control
Median Coverage ≥ 300X 
Mutation Detection Sensitivity 10%
Hands on Time ≤ 3.5 hours
Turn around Time 2.5 days including overnight sample incubation
Pack Size 4x16

PRODUCT FLYERS

Sentosa Oncology Panels Flyer RUO EN

ORDERING INFORMATION

ProductPack SizeItem Number
Sentosa SQ OncoKey Select Reagents (4x16) RUO 4x16 300692
Sentosa SQ OncoKey Solutions (4x16) RUO 4x16 300693

For availability in your country, please contact your Vela Diagnostics sales representative. Availability is subject to country's regulatory approval.

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