Sentosa® SQ OncoKey Focus

Detect sequence variants in non-small cell lung cancer (NSCLC) and colorectal cancer (CRC) samples

INTENDED USE

Sentosa SQ OncoKey Focus Automated Workflow

The Sentosa SQ OncoKey Focus Panel is a next-generation sequencing (NGS)-based test to detect single nucleotide variants (SNVs) and insertions/deletions (indels) in formalin-fixed paraffin-embedded (FFPE) samples.

This test is intended to be used on the Sentosa SX101 instrument, in conjunction with the Sentosa SX FFPE II gDNA Kit and Sentosa SQ301 and Sentosa ST401 instruments as an automated workflow. Up to 15 samples can be automatically processed and analysed within 2.5 days. The Sentosa SQ OncoKey Focus Panel is for research use only (RUO) and not for use in diagnostic procedures.


Sentosa SQ OncoKey Focus Manual Workflow

The Sentosa SQ OncoKey Focus Manual Library Set provides the users a manual solution. This test is composed of the Sentosa SQ OncoKey Focus Library Kit, Sentosa SQ Barcode Kit 1-16 and Sentosa SQ OncoKey Solution Prep Kit for manual library preparation with extracted gDNA samples. It is compatible with the Sentosa SQ301 and Sentosa ST401 instruments and Ion Torrent sequencing platforms. The Sentosa SQ OncoKey Focus Manual Library Set is for research use only (RUO) and not for use in diagnostic procedures.

TARGET GENES

31 targeted genes
AKT1 FBXW7 NOTCH1
ALK FGFR1 NRAS
APC FGFR2 PIK3CA
BRAF FGFR3 PTEN*
CDKN2A GNAS RET
CTNNB1 KRAS ROS1
DDR2 MAP2K1 SMAD4
EGFR MAP3K1 SMO
ERBB2 MET STK11*
ERBB4 MTOR TP53*
KIT    

       *Hotspot

FEATURES AND BENEFITS

  • Detect 5% variant frequency for mutation
  • Sample input: 5-10 µm section of FFPE sample
  • From samples to analysed results in 2.5 days
  • Combine different solid tumor types in one run
  • The Sentosa SQ OncoKey Focus Manual Library Set can be used on different NGS instruments
  • Pay per gene: pay only for your selected genes of interest
  • 16 different barcoded DNA Adapters, each sufficient for six libraries
  • Seamless sample traceability and IT connectivity
  • Flexible manual solution or automated workflow with 4 hours of hands-on time available

WORKFLOW

SPECIFICATIONS (AUTOMATED WORKFLOW)

Attribute Sentosa SQ OncoKey Focus Panel
Regulatory Status RUO
Targeted Cancer NSCLC, CRC
Number of Genes 31
Number of Amplicons ~130
Target Mutations ~384
Sample Type FFPE
Sample Input 10 µm section of FFPE sample with > 10% tumor content
Sample Throughput Up to 15 samples + 1 system control
Median Coverage ≥ 500X 
Mutation Detection Sensitivity 5% - 10%
Hands on Time ≤ 4 hours
Turn around Time 2.5 days including overnight sample incubation
Pack Size 4x16

PRODUCT FLYERS

Sentosa Oncology Panels Flyer RUO EN

ORDERING INFORMATION

ProductPack SizeItem Number
Sentosa SQ OncoKey Focus Library Kit (96) RUO 96 300711
Sentosa SQ OncoKey Focus Reagents (4x16) RUO 4x16 300730
Sentosa SQ Barcode Kit 1-16 (96) RUO 1x96 300714
Sentosa SQ OncoKey Solution Prep Kit (96) RUO 96 300713
Sentosa SQ OncoKey Solutions (4x16) RUO 4x16 300693
VELA Analytics Software 480175

For availability in your country, please contact your Vela Diagnostics sales representative. Availability is subject to country's regulatory approval.

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