OncoKey SL 60 Plus

Unlock Pan-Cancer Molecular Information

PURPOSE

OncoKey SL 60 Plus on Automated Workflow

The Oncokey SL 60 Plus Auto is a Next-Generation sequencing (NGS)-based Research Use Only (RUO) test intended for the detection of single nucleotide variations (SNVs), insertions/deletions (INDELs), copy number variations (CNVs), microsatellite instability (MSI), oncogenic pathogens and fusion genes in 60 Plus targeted genes, in Formalin Fixed Paraffin Embedded (FFPE) Tissue specimens. 

This test is automated on the Sentosa SX 101 with the OncoKey SX FFPE DNA/RNA Kit (4x16), OncoKey SL DNA Library Prep Kit (4x16), OncoKey SL RNA Library Prep Kit, Oncokey SL 60 Plus DNA/RNA Target Enrichment Kit, in conjunction with Illumina® Miseq or NextSeq systems.

OncoKey SL 60 Plus on Manual Workflow

The OncoKey SL 60 Plus is also available as a manual solution.

This manual test is intended to be used with the OncoKey SX FFPE DNA/RNA Kit (4x16), OncoKey SL Manual DNA Library Prep Kit, OncoKey SL Manual RNA Library Prep Kit, Oncokey SL 60 Plus Manual DNA/RNA 8-Plex Target Enrichment Kit, in conjunction with Illumina® Miseq or NextSeq systems.

* RUO: For research use only. Not for use in diagnostic procedures.

 

TARGET GENES

FEATURES AND BENEFITS

Supporting Clinical Research:

  • Pan-cancer test covering more than 60 clinically relevant genes
  • Detecting multiple DNA & RNA targets in one run:
  • SNVs, Fusions, Microsatellite Instability (MSI), CNVs, Indels, Oncogenic Viruses and Bacteria
  • From clinical samples to result in 5 days

Providing Laboratory Efficiency:

  • Up to 32 samples (Illumina Sequencing Platform dependent)
  • Flexible automated workflow or manual solution with 5h hands-on time available
  • Seamless sample traceability and IT connectivity
  • Automated comprehensive analysis and drug interpretation report by Vela Analytics

 

ONCOKEY AUTOMATED WORKFLOW

SPECIFICATIONS (AUTOMATED WORKFLOW)

Attribute OncoKey SL 60 Plus Panel
Regulatory Status RUO
Genomic Coverage 266 kb (Human)
82.7 kb (Oncogenic Pathogens)
Systems MiSeq System, NextSeq System and above
DNA Input Requirement 40 ng DNA
RNA Input Requirement 40 ng RNA
FFPE Input Requirement Min. 0.5mm3, 3 x 10 μm FFPE scrolls/slides
Median Coverage > 200x
Sample Throughput 8 samples per MiSeq run
32 samples per NextSeq run
Hands-on Time ≤ 5 hours
Turnaround Time 5 days from sample to result
Limit of Detection 5% DNA VAF
Analytical Sensitivity DNA: 100%
RNA: > 96%
Analytical Specificity DNA: 100%
RNA: > 96%

PRODUCT FLYERS

OncoKey SL 60 Plus  RUO EN

 

ORDERING INFORMATION

ProductPack SizeItem Number
OncoKey SL 60 Plus DNA 8-Plex Target Enrichment Reagents (4x2) (RUO) 4x2 301031
OncoKey SL 60 Plus DNA/RNA 8-Plex Target Enrichment Reagents (4x4) (RUO) 4x4 301032
OncoKey SL 60 Plus Manual DNA Target Enrichment Reagents (24) (RUO) 24 301047
OncoKey SL 60 Plus Manual DNA/RNA 8-Plex Target Enrichment Reagents (24) (RUO) 24 301048
OncoKey SL 60 Plus Manual RNA Target Enrichment Reagents (24) (RUO) 24 301062
OncoKey SL 60 Plus RNA 8-Plex Target Enrichment Reagents (4x2) (RUO) 4x2 301065
OncoKey SL 8-Plex Target Enrichment Solutions (4x2) (RUO) 4x2 301037
OncoKey SL 8-Plex Target Enrichment Solutions (4x4) (RUO) 4x4 301038
OncoKey SL DNA Library Prep Reagents (4x16) (RUO) 4x16 301027
OncoKey SL DNA Library Prep Solutions (4x16) (RUO) 4x16 301028
OncoKey SL Internal Control Kit (8) (RUO) 8 301024
OncoKey SL Manual 8-Plex Target Enrichment Solutions (24) (RUO) 24 301053
OncoKey SL Manual DNA Library Prep Adapters 1-32 (96) (RUO) 96 301041
OncoKey SL Manual DNA Library Prep Adapters 33-64 (96) (RUO) 96 301042
OncoKey SL Manual DNA Library Prep Reagents (96) (RUO) 96 301039
OncoKey SL Manual DNA Library Prep Solutions (96) (RUO) 96 301040
OncoKey SL Manual RNA Library Prep Adapters 65-96 (96) (RUO) 96 301045
OncoKey SL Manual RNA Library Prep Adapters 97-128 (96) (RUO) 96 301046
OncoKey SL Manual RNA Library Prep Reagents (96) (RUO) 96 301043
OncoKey SL Manual RNA Library Prep Solutions (96) (RUO) 96 301044
OncoKey SL Positive Control Kit (8) (RUO) 8 301023
OncoKey SL RNA Library Prep Reagents (4x16) (RUO) 4x16 301029
OncoKey SL RNA Library Prep Solutions (4x16) (RUO) 4x16 301030
Sentosa SX FFPE DNA/RNA Kit Box 1 (4x16) (RUO) 4x16 301025
Sentosa SX FFPE DNA/RNA Kit Box 2 (4x16) (RUO) 4x16 301026

For availability in your country, please contact your Vela Diagnostics sales representative. Availability is subject to country's regulatory approval.

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