OncoKey SL 525 Plus Panel

Streamlining Precision Medicine for Cancer

PURPOSE

OncoKey SL 525 Plus Panel on an Automated Workflow

The Oncokey SL 525 Plus Panel is a Next-Generation sequencing (NGS)-based Research Use Only (RUO) test intended for the detection of single nucleotide variations (SNVs), insertions/deletions (INDELs), copy number variations (CNVs), microsatellite instability (MSI), oncogenic pathogens, fusion genes and tumour mutation burden (TMB) in clinically relevant genes, in Formalin Fixed Paraffin Embedded (FFPE) Tissue specimens. 

This test is automated on the Sentosa SX 101 with the OncoKey SX FFPE DNA/RNA Kit (4x16), OncoKey SL DNA Library Prep Kit (4x16), OncoKey SL RNA Library Prep Kit, Oncokey SL 525 Plus DNA/RNA Target Enrichment Kit, in conjunction with Illumina NextSeq 500/550 or NovaSeq 6000 systems.

OncoKey SL 525 Plus Panel on a Manual Workflow

The OncoKey SL 525 Plus Panel is also available as a manual solution.

This manual test is intended to be used with the OncoKey SX FFPE DNA/RNA Kit (4x16), OncoKey SL Manual DNA Library Prep Kit (96), OncoKey SL Manual RNA Library Prep Kit (96), Oncokey SL 525 Plus Manual DNA/RNA 8-Plex Target Enrichment Kit (24), in conjunction with Illumina, NextSeq 500/550 or NovaSeq 6000 systems.

*RUO: For research use only. Not for use in diagnostic procedures.

CANCER GENES

TARGET GENES

FEATURES AND BENEFITS

Supporting Clinical Research:

  • Comprehensive test covering more than 525 cancer-related genes
  • Detects multiple cancer biomarkers in one run:
    • SNVs, CNVs, INDELs, MSI, fusions, splice variants
    • Oncogenic viruses and bacteria
    • TMB
  • Optimal sensitivity, specificity, variant detection and sequencing coverage uniformity
  • FFPE positive control, internal control and RNA housekeeping genes are included
  • Local data storage to preserve patient data privacy

Providing Laboratory Efficiency:

  • Up to 64 samples (Illumina sequencing platform dependent)
  • Automated, sample-to-result workflow
  • Generates results within 5 days, and less than 2.5 hours of hands-on time
  • Manual option available for lower sample volumes
  • Seamless sample traceability
  • Automated comprehensive analysis and drug interpretation report by VELA Analytics

AUTOMATED WORKFLOW

SPECIFICATIONS (AUTOMATED WORKFLOW)

Attribute OncoKey SL 525 Plus Panel
Regulatory Status RUO
Genomic Coverage 1.89 Mb (Human)
82.7 kb (Oncogenic Pathogens)
Systems NextSeq 500/550 System & NovaSeq 6000 System
DNA Input Requirement 40 ng DNA
RNA Input Requirement 40 ng RNA
FFPE Input Requirement Tumor content > 20%, 3 x 10 μm FFPE scrolls/slides
Median Coverage > 200x
Sample Throughput 8 samples per NextSeq 500/550 run
16-64 samples per NovaSeq 6000 run
Hands-on Time ≤ 2.5 hours
Turnaround Time 5 days from sample to result
Limit of Detection 5% DNA VAF
Analytical Sensitivity DNA: 100%
RNA: 100%
Analytical Specificity DNA: 100%
RNA: 100%

PRODUCT FLYERS

OncoKey SL 525 Plus Panel RUO EN

 

ORDERING INFORMATION

ProductPack SizeItem Number
Sentosa SX NGS Starter Kit 400015
Sentosa SX101 1 400089
OncoKey SL 525 Plus DNA 8-Plex Target Enrichment Reagents 4x2 301035
OncoKey SL 525 Plus DNA/RNA 8-Plex Target Enrichment Reagents 4x4 301036
OncoKey SL 525 Plus Manual DNA 8-Plex Target Enrichment Reagents 24 301051
OncoKey SL 525 Plus Manual DNA/RNA 8-Plex Target Enrichment Reagents 24 301052
OncoKey SL 525 Plus Manual RNA 8-Plex Target Enrichment Reagents 24 301064
OncoKey SL 525 Plus RNA 8-Plex Target Enrichment Reagents 4x2 301066
OncoKey SL 8-Plex Target Enrichment Solutions 24 301053
OncoKey SL 8-Plex Target Enrichment Solutions 4x2 301037
OncoKey SL 8-Plex Target Enrichment Solutions 4x4 301038
OncoKey SL DNA Library Prep Reagents 4x16 301027
OncoKey SL DNA Library Prep Solutions 4x16 301028
OncoKey SL Internal Control Kit 8x16 301024
OncoKey SL Manual DNA Library Prep Adapters 1-32 96 301041
OncoKey SL Manual DNA Library Prep Adapters 33-64 96 301042
OncoKey SL Manual DNA Library Prep Reagents 96 301039
OncoKey SL Manual DNA Library Prep Solutions 96 301040
OncoKey SL Manual RNA Library Prep Adapters 65-96 96 301045
OncoKey SL Manual RNA Library Prep Adapters 97-128 96 301046
OncoKey SL Manual RNA Library Prep Reagents 96 301043
OncoKey SL Manual RNA Library Prep Solutions 96 301044
OncoKey SL Positive Control Kit 8 301023
OncoKey SL RNA Library Prep Reagents 4x16 301029
OncoKey SL RNA Library Prep Solutions 4x16 301030
SQ Reporter Server for OncoKey SL Panels 400254
Sentosa SQ Reporter v2.5 480204
Sentosa SX FFPE DNA/RNA Kit Box 1 4x16 301025
Sentosa SX FFPE DNA/RNA Kit Box 2 4x16 301026
Sentosa SX Magnetic Plate 400090
Sentosa SX Thermoblock PCR 96 OC 400088
VELA Analytics Report OncoKey SL Plus 500260
VELA Analytics Server, T620 400102
VELA Analytics Software 480175
VELA SL Wingman 480182

For availability in your country, please contact your Vela Diagnostics sales representative. Availability is subject to country's regulatory approval.

For more product related documents, please contact us. 

DOWNLOADS



User Manual
OncoKey SL Plus Panels Technical Note V1.0
OncoKey SX FFPE DNA-RNA Kit (Manual) (4x16) (RUO) V4.0
OncoKey SL Plus Panels V2.1
OncoKey SL Manual DNA Library Prep (96) (RUO) V2.0
OncoKey SL Manual RNA Library Prep (96) (RUO) V2.0
OncoKey SL Manual Target Enrichment (24) (RUO) V3.0
400089 Sentosa SX101 (IVD) V2.0
480175 VELA Analytics Software (RUO) V2.0


MSDS
OncoKey SL Positive Control Kit (8) (RUO) V1.0
OncoKey SL Internal Control Kit (8) (RUO) V1.0
OncoKey SX FFPE DNA-RNA Kit Box 1 (4x16) V2.0
OncoKey SX FFPE DNA-RNA Kit Box 2 (4x16) V2.0
OncoKey SL DNA Library Prep Reagents (4x16) V1.0
OncoKey SL DNA Library Prep Solutions (4x16) V1.0
OncoKey SL RNA Library Prep Reagents (4x16) V1.0
OncoKey SL RNA Library Prep Solutions (4x16) V1.0
OncoKey SL 525 Plus DNA/RNA 8-Plex Target Enrichment Reagents (4x4) RUO V1.0
OncoKey SL 8-Plex Target Enrichment Solutions (4x4) RUO V1.0
OncoKey SL 525 Plus DNA 8-Plex Target Enrichment Reagents (4x2) RUO V1.0
OncoKey SL 8-Plex Target Enrichment Solutions (4x2) RUO V1.0
OncoKey SL 525 Plus RNA 8-Plex Target Enrichment Reagents (4x2) RUO V1.0
OncoKey SL Manual DNA Library Prep Reagents (96) RUO V2.0
OncoKey SL Manual DNA Library Prep Solutions (96) RUO V2.0
OncoKey SL Manual DNA Library Prep Adapters 1-32 (96) RUO V2.0
OncoKey SL Manual DNA Library Prep Adapters 33-64 (96) RUO V2.0
OncoKey SL Manual RNA Library Prep Reagents (96) RUO V2.0
OncoKey SL Manual RNA Library Prep Solutions (96) RUO V2.0
OncoKey SL Manual RNA Library Prep Adapters 65-96 (96) RUO V2.0
OncoKey SL Manual RNA Library Prep Adapters 97-128 (96) RUO V2.0
OncoKey SL 525 Plus Manual DNA/RNA 8-Plex Target Enrichment Reagents (24) RUO V1.0
OncoKey SL Manual 8-Plex Target Enrichment Solutions (24) RUO V2.0
OncoKey SL 525 Plus Manual DNA Target Enrichment Reagents (24) RUO V1.0
OncoKey SL 525 Plus Manual RNA Target Enrichment Reagents (24) RUO V1.0


Product Info
OncoKey SL Positive Control Kit (8) (RUO) V1.1
OncoKey SL Internal Control Kit (8) (RUO) V1.1
301025 OncoKey SX FFPE DNA-RNA Kit Box 1 (4x16) (RUO) V3.0
301026 OncoKey SX FFPE DNA-RNA Kit Box 2 (4x16) (RUO) V3.0
OncoKey SL DNA Library Prep Reagents (4x16) V1.0
OncoKey SL DNA Library Prep Solutions (4x16) V1.0
OncoKey SL RNA Library Prep Reagents (4x16) V1.0
OncoKey SL RNA Library Prep Solutions (4x16) V1.0
OncoKey SL 525 Plus DNA/RNA 8-Plex Target Enrichment Reagents (4x4) RUO V1.0
OncoKey SL 8-Plex Target Enrichment Solutions (4x4) RUO V1.0
OncoKey SL 525 Plus DNA 8-Plex Target Enrichment Reagents (4x2) RUO V1.0
OncoKey SL 8-Plex Target Enrichment Solutions (4x2) RUO V1.0
OncoKey SL 525 Plus RNA 8-Plex Target Enrichment Reagents (4x2) RUO V1.0
OncoKey SL Manual DNA Library Prep Reagents (96) (RUO) V2.0
OncoKey SL Manual DNA Library Prep Solutions (96) (RUO) V2.0
OncoKey SL Manual DNA Library Prep Adapters 1-32 (RUO) V2.0
OncoKey SL Manual DNA Library Prep Adapters 33-64 (RUO) V2.0
OncoKey SL Manual RNA Library Prep Reagents (96) (RUO) V2.0
OncoKey SL Manual RNA Library Prep Solutions (96) (RUO) V2.0
OncoKey SL Manual RNA Library Prep Adapters 65-96 (96) (RUO) V2.0
OncoKey SL Manual RNA Library Prep Adapters 97-128 (RUO) V2.0
OncoKey SL 525 Plus Manual DNA/RNA 8-Plex Target Enrichment Reagents (24) RUO V1.0
OncoKey SL Manual 8-Plex Target Enrichment Solutions (24) RUO V3.0
OncoKey SL 525 Plus Manual DNA Target Enrichment Reagents (24) RUO V1.0
OncoKey SL 525 Plus Manual RNA Target Enrichment Reagents (24) RUO V1.0