PathoKey SQ FLEX Pathogen ID Assay

Qualitative Detection of Pathogens from Complex Microbiome Samples

PURPOSE

PathoKey SQ FLEX Pathogen ID Assay

The PathoKey SQ FLEX Pathogen ID Assay (4x24) is a next generation sequencing-based test that provides comprehensive and robust profiling of bacterial, fungal, eukaryotic communities and viruses—from sources such as gut microbiome, contaminated food and clinical samples respectively.

It has the flexibility to interrogate any combination of bacterial 16S hypervariable regions (V1-V2 and V3-V5), and rpoB regions, fungal hypervariable regions (ITS1 and ITS2), and 28S region (D2), and eukaryotic community 18S hypervariable regions (V3 and V8-V9), and 28S region (D2). In addition, the assay detects up to 10 viruses and 32 AMR genes.

This test is intended to be used in conjunction with the Sentosa SX Lysis Plate Type B (10x24) and Sentosa SX U-Prep Kit (4) on Sentosa SX101, Sentosa SQ 318 Chip Kit and Sentosa ST Template Kit, Sentosa SQ Sequencing Kit on Sentosa ST401 and Sentosa SQ301 instrument, and Sentosa SQ Reporter v2.3.

FEATURES AND BENEFITS

Unparalleled Versatility

  • 5 panels to choose from:  bacteria, fungi, parasites, viruses and genotypic antimicrobial resistance (AMR)
  • Amplicon sequencing allows for identification of up to 4 groups in one run

Comprehensive Detection

  • Compares all detected pathogens to a database of >500,000 known sequences
  • Relative abundances are reported, allowing for differentiation of commensals or environmental contamination vs pathogenic microbes

Maximizing Laboratory Efficiency

  • No need to spend extended periods of time culturing samples
  • Sample-to-results in 2 days, with less than 2 hours of hands-on-time
  • Highly automated NGS workflow that is easy to adopt, with automated data analysis and report generation with Sentosa SQ Reporter

AUTOMATED WORKFLOW

SPECIFICATIONS

Sample Types* Serum, stool, nasopharyngeal swabs, skin, bronchoalveolar lavage (BAL), cerebrospinal fluid (CSF), plasma, whole blood, FFPE, saliva, urine
Sample Throughput 22 samples per run
Turnaround Time ~ 2 days (from sample to results)
Hands-On Time 65-100 minutes
Controls 1 positive control, 1 negative control, 1 extraction control
Targets

Bacteria: V1-V2 and V3-V5 hypervariable region in 16S, and rpoB gene

Fungi: ITS1 and ITS2 hypervariable region, and D2 region in 28S

Eukaryotes: V3 and V8-V9 hypervariable region in 18S, and D2 region in 28S

Viruses: 10 types of viruses

AMR: 32 AMR genes 

Number of Detectable Pathogens

>2000

*Some sample types may require pre-treatment.

PRODUCT FLYERS

PathoKey SQ FLEX Pathogen ID Assay Flyer RUO EN

ORDERING INFORMATION

ProductPack SizeItem Number
Sentosa SQ Virus Testing Solutions (RUO) 4x24 300775
PathoKey SQ FLEX AMR ID Kit (96) (RUO) 4x24 301083
PathoKey SQ FLEX Bacteria ID Kit (96) (RUO) 4x24 301079
PathoKey SQ FLEX Fungi ID Kit (96) (RUO) 4x24 301080
PathoKey SQ FLEX Library Prep Reagents (4x24) (RUO) 4x24 301078
PathoKey SQ FLEX Parasite ID Kit (96) (RUO) 4x24 301081
PathoKey SQ FLEX Pathogen Positive Control (4) (RUO) 4 301084
PathoKey SQ FLEX Virus Panel I ID Kit (96) (RUO) 4x24 301082
PathoKey SX Lysis Plate Type B (10x24) 4x16 300792
PathoKey SX U-Prep Kit (4) 4 runs 300836

For availability in your country, please contact your Vela Diagnostics sales representative. Availability is subject to country's regulatory approval.

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