Determination of Tropical Infections


Detect and differentiate 3 different mosquito-bourne viruses in a single sample with the Sentosa® SA
ZIKV-DENV-CHIKV RT-PCR Test. Our real-time PCR assay enables you to identify the presence of Zika, Dengue,
and Chikungunya virus in under 3 hours with only 250 uL of sample input required. 

Simultaneous Detection and Dierentiation of Tropical Viruses
As presented at the 2016 American Society of Tropical Medicine and Hygeine, co-infection of Dengue,
Chikungunya and Zika may occur with a single Aedes mosquito bite. These three viruses present similar
symptoms, but yield different outcomes, and furthermore, the presence of multiple viruses may produce
even greater clinical impact. In order to further understand these viral infections, more clinical research is
necessary. The Sentosa® SA ZIKV-DENV-CHIKV RT-PCR Assay enables a simple, single workflow to detect and
differentiate these viruses from a single sample.

RUO: For Research Use Only.



  • Single multiplex test enables detection of Zika, Dengue, and Chikungunya including 82 strains of Zika and all 4 serotypes of Dengue
  • HIgh analytical performance with no cross-reactivity
  • Low detection limit enables detection of samples with low viral load, only 250 uL of sample input needed
  • Automated solution delivers results in about 3 hours



Target Gene ZIKV MR-766 and ZIKV PRAVBC59 3'UTR E1 gene
Analytical Sensitivity (95% CI) ZIKV MR-766: 6 x 103 copies/mL
ZIKV PRAVBC59: 3 x 103 copies/mL 

9 pfu/ml;7.5 copies/uL

31.0 pfu/mL; 9.0 copies/uL
Analytical Reactivity 82 strains tested & detected 4 strains tested & detected 1 strain tested & detected
Analytical Specificity No cross reactivity with 44 pathogens tested No cross reactivity with 13 pathogens tested No cross reactivity with 16 pathogens tested
Sample Type(s) EDTA plasma, serum or urine (collected alongside a patient-matched EDTA plasma and / or serum specimen) EDTA plasma and serum EDTA plasma and serum
Test combination  One sample required to run all 3 tests
Controls Postivite control (PC), Negative control (NC), & Extraction Control (EC)
Automation Lysis, extraction & PCR setup


ProductPack SizeItem Number
Sentosa SX Virus Total Nucleic Acid Kit v2.0 (IVD) 4x24 300353

For availability in your country, please contact your Vela Diagnostics sales representative.