Sentosa SA NRAS PCR Test

Detection of NRAS mutations

DESCRIPTION

The Sentosa SA NRAS PCR Test is a real-time polymerase chain reaction (PCR) based research use only test for the qualitative detection of mutations in the NRAS gene at Q61 position. It detects Q61K, Q61L and Q61R mutations in formalin-fixed, paraffin-embedded (FFPE) samples.

This research use only test is configured for use with the Sentosa SX101 nucleic acid extraction platform, with the Sentosa SX FFPE gDNA Kit (4x8), in conjunction with the Rotor-Gene® Q MDX 5plex HRM instrument.

For Research Use Only.  Not for use in diagnostic procedures.

FEATURES AND BENEFITS

Supporting Research

  • LoD of 10% tumor content in WT DNA for the detection of NRAS mutations
  • Covers the most frequent NRAS mutations in the Catalogue of Somatic Mutations in Cancer (COSMIC)

Providing Laboratory Effectiveness

  • Internal controls to monitor DNA extraction, PCR efficiency, and monitor PCR inhibition
  • Minimal sample DNA requriements; no macrodissection necessary
  • Automated sample extraction reduces labor cost and time to results

Increasing Cost Effectiveness

  • Single runs 
  • Less than 15% of entire workflow require hands-on time

WORKFLOW

ORDERING INFORMATION

ProductPack SizeItem Number
Sentosa SA NRAS PCR Test 4x8 300135
Sentosa SX FFPE gDNA Kit 4x8 300051
Sentosa SX101 1 400089
Rotor-Gene® Q MDx 1 400046