Sentosa SA BRAF V600 PCR Test

Detection of BRAF mutations


The Sentosa SA BRAF V600 PCR Test is a real-time polymerase chain reaction (PCR) based research use only test for the qualitative detection of mutations in the BRAF gene. It detects both V600E and V600K mutations in formalin-fixed, paraffin-embedded (FFPE) samples.

This research use only test is configured for use with the Sentosa SX101 nucleic acid extraction platform, with the Sentosa SX FFPE gDNA Kit, in conjunction with the Rotor-Gene® Q MDX 5plex HRM instrument.

For Research Use Only.  Not for use in diagnostic procedures.


Supporting Research

  • LoD of 10% tumor content in WT DNA for the detection of BRAF mutations
  • Covers the most frequent BRAF mutations in the Catalogue of Somatic Mutations in Cancer (COSMIC)

Providing Laboratory Effectiveness

  • Internal controls to monitor DNA extraction, PCR efficiency, and monitor PCR inhibition
  • Minimal sample DNA requriements; no macrodissection necessary
  • Automated sample extraction reduces labor cost and time to results

Increasing Cost Effectiveness

  • Single runs 
  • Less than 15% of entire workflow require hands-on time



ProductPack SizeItem Number
Sentosa SA BRAF V600 PCR Test Kit (RUO) 4x8 300129
Sentosa SX FFPE gDNA Kit (RUO) 4x8 300051
Sentosa SX101 1 400089
Rotor-Gene® Q MDx 1 400046