Rotor-Gene® Q MDx

5plex HRM platform

OVERVIEW

Using an innovative rotary design, the Rotor-Gene® Q MDx 5plex HRM Platform allows for unmatched thermal performance in real-time PCR cycling. The use of a rotating disc ensures that every single tube is spun in a chamber of uniform temperature, using air as the conducting element. This allows for rapid thermal cycling and precise results in all tubes.

For detection, a set of 6 uncoupled excitation and emission filters are used in conjunction with 6 dedicated LEDs to maximize the detection capability. Each tube is illuminated by the excitation LED and the fluorescent signal transmitted to the PMT detector via a uniform optical path length. This eliminates well-to-well optical variation due to edge effect which block-based cycler had experienced. It also allows for fluorescent detection without the need of a reference dye.

With thermal and optical uniformity, the Rotor-Gene® Q MDx 5plex HRM Platform offers unparalleled quantitative and genotyping analysis for PCR applications.

OPTICAL RANGE

With 5 channels offering detection from the green to infrared wavelengths, the Rotor-Gene® Q MDx platform provides wide optics for most fluorophores available in the market. The decoupled filter sets create flexibility for customized excitation/detection wavelength combinations, making the platform compatible with most fluorophores.

ChannelExcitation (nm)Detection (nm)Examples of fluorophores detected
Green 470±10 510±5 FAM, YYBR Green I, Fluorescein, EvaGreen, Alexa Fluor 488
Yellow 530±5 557±5 JOE, VIC, HEX, TET, MAX, CAL Fluor Gold 540, Yakima Yellow
Orange 585±5 610±5 ROX, CAL Fluor Red 610, Cy 3.5, Texas Red, Alexa Fluor 568
Red 625±5 660±10 Cy5, Quasar 670, LightCycler Red640, Alexa Fluor 663
Crimson 680±5 712 high pass Quasar 705, LightCycler Red 705, Alexa Fluor 680
High Resolution Melt (HRM) 460±20 510±5 SYBR Green I, SYTO 9, LC Green, LC Green Plus+, EvaGreen

FEATURES AND BENEFITS

  • Works in combination with Sentosa SX101 for upstream sample extraction and PCR set-up
  •  
  • Thermal non-uniformity of ±0.02°C
  • 5 sets of decoupled excitation/emission filters supporting a wide variety of dyes
  • Optical uniformity allows PCR without the need of reference dye
  • Rapid thermal heating and cooling using air as conducting element
  • Optically stable LEDs with life time guarantee
  • No optical calibration required during installation
  • “Spin down” of tubes is not required
  • Optical Temperature Verification (OTV) kit available for simple thermal verification for performance without the need for contacting a service technician
  • Easy to clean, low maintenance & service requirement
  • Small footprint (W37;H27.5;D42 cm) (W14.6;H10.8;D16.5 in)

ORDERING INFORMATION

ProductPack SizeItem Number
Sentosa SX qPCR Starter Kit 1 400107
Sentosa SX101 1 400089
Rotor-Gene® Q MDx 1 400046