Sentosa SQ OncoKey Focus

Detect sequence variants in NSCLC and CRC samples

INTENDED USE

SQ OncoKey Focus

The Sentosa SQ OncoKey Focus Panel is a Next-Generation Sequencing (NGS)-based assay to detect single nucleotide variants (SNVs) and deletions in formalin-fixed paraffin-embedded (FFPE) samples.

The Sentosa SQ OncoKey Focus panel is intended to be used on the Sentosa SX101, the Sentosa SX FFPE II gDNA Kit and the Sentosa SQ301 instruments as an automated workflow. 15 samples can be automatically processed and analysed within 2.5 days. The Sentosa SQ OncoKey Focus panel is for research use only (RUO) and not for use in diagnostic procedures.


SQ OncoKey Focus Manual Library Set

The same OncoKey Focus panel design is also available as Sentosa SQ OncoKey Focus Manual Library Set (96) which gives the users a manual solution.

The Sentosa SQ OncoKey Focus Manual Library Set (96) is composed of the Sentosa SQ OncoKey Focus Library Kit, Sentosa SQ Barcode Kit 1-16 (96) and the Sentosa SQ OncoKey Solution Prep Kit (96) for manual library preparation with extracted gDNA samples. It is compatible with the Sentosa SQ 301 instruments and the Ion Torrent™ sequencing platforms. The Sentosa SQ OncoKey Focus Manual Library Set is for research use only (RUO) and not for use in diagnostic procedures.

TARGET GENES

OncoKey Focus 30 targeted genes
AKT1 FBXW7 NOTCH1
ALK FGFR1 NRAS
APC FGFR2 PIK3CA
BRAF FGFR3 PTEN*
CDKN2A GNAS RET
CTNNB1 KRAS ROS1
DDR2 MAP2K1 SMAD4
EGFR MAP3K1 SMO
ERBB2 MET STK11*
ERBB4 MTOR TP53*

       *Hotspot

FEATURES AND BENEFITS

  • Detect 5% variant frequency for mutation
  • Sample input: 5-10 µm section of FFPE sample
  • From samples to analysed results in 2.5 days*
  • Combine different solid tumor types in one run
  • The OncoKey Focus Manual Library Set can be used on different NGS instruments
  • Pay per gene: pay only for your selected genes of interest*
  • 16 different barcoded DNA Adapters, each sufficient for 6 libraries
  • Seamless sample traceability and IT connectivity with Sentosa Link*
  • Flexible manual solution or automated workflow with 4h hands on time time available

* Does not apply for the OncoKey Focus Manual Library Set 

WORKFLOW

SPECIFICATIONS (AUTOMATED WORKFLOW)

Attribute Sentosa SQ OncoKey Focus Panel
Regulatory Status RUO
Targeted Cancer NSCLC, CRC
Number of Genes 30
Number of Amplicons ~130
Target Mutations ~301
Sample Type FFPE
Sample Input 10 µm section of FFPE sample with > 10% tumor content
Sample Throughput Up to 15 samples + 1 system control
Median Coverage ≥ 500X 
Mutation Detection Sensitivity 5% - 10%
Hands on Time ≤ 4 hours
Turn around Time 2.5 days including overnight sample incubation
Pack Size 4x16

ORDERING INFORMATION

ProductPack SizeItem Number
Sentosa SQ 318 Chip Kit (RUO) 8 runs 300301
Sentosa SQ Barcode Kit 1-16 1x96 300714
Sentosa SQ OncoKey Focus Library Kit (RUO) 96 300711
Sentosa SQ OncoKey Focus Reagents (RUO) 4x16 300730
Sentosa SQ OncoKey Solution Prep Kit (RUO) 96 300713
Sentosa SQ OncoKey Solutions (RUO) 4x16 300693
Sentosa SQ Sequencing Reagents (RUO) 300310
Sentosa SQ Sequencing Solutions (RUO) 300314
Sentosa SQ Sequencing Supplies (RUO) 300304
Sentosa ST Template Beads (RUO) 300320
Sentosa ST Template Reagents (RUO) 300317
Sentosa ST Template Solutions (RUO) 300323
Sentosa ST Template Supplies (RUO) 300326
Sentosa SX FFPE II gDNA Kit (RUO) 4x8 tests 300231

For availability in your country, please contact your Vela Diagnostics sales representative. Availability is subject to country's regulatory approval.

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