Sentosa SQ OncoKey Core

Detect sequence variants in NSCLC, CRC, Thyroid and Melanoma samples

INTENDED USE

SQ OncoKey Core on Automated Workflow

The Sentosa SQ OncoKey Core is a family of panels consisting of four panels: A non-small cell lung cancer (NSCLC) panel, a colorectal cancer (CRC) panel, thyroid panel and a melanoma panel. All panels are Next-Generation Sequencing (NGS) tests. All tests are automated on the Sentosa workflow.

The Sentosa SQ OncoKey Core is intended to be used on the Sentosa SX101, the Sentosa SX FFPE II gDNA Kit, Sentosa ST401 and Sentosa SQ301 instruments. On this automated workflow 7 samples can be automatically processed and analysed within 2.5 days.

The Sentosa SQ OncoKey Core is for research use only (RUO), and not for use in diagnostic procedures.

 

SQ OncoKey Core Primer Set on Manual Workflow

The same OncoKey Core design for the NSCLC/CRC/Thyroid panel is also available as Sentosa SQ OncoKey Core Primer Set (96) which gives users a manual solution.

The Sentosa SQ OncoKey Core Primer Set contains the Core Primer pool, the OncoKey System Control and barcoded DNA adapters sufficient for up to 16 libraries per run and 96 in total. The Sentosa SQ OncoKey Core Primer Set is for research use only (RUO), and not for use in diagnostic procedures.

Sentosa SQ OncoKey Core Primer Set is designed to be used with the Ion AmpliSeq™ DNA Library Preparation kits for multiplex targeted libraries from DNA for sequencing on Sentosa SQ301 or the Ion PGM/S5/S5 XL systems, following the manufacturer's instructions for the details of the procedure.

 

TARGET GENES

 

 

Target Genes

NSCLC

Number of

Target Mutations

Amplicon

Locations in Exon(s)

NRAS 19 2, 3, 4
CTNNB1 5 3
PIK3CA 14 11, 22
FGFR3 9 7, 9, 14
KIT 8 11, 13, 17
EGFR 10 18, 19, 20, 21
BRAF 15 11, 15
RET 1 16
PTEN 3 5, 7
KRAS 22 2, 3, 4
TP53 7 4, 6, 7
Total 113  

 

 

Target Genes

CRC

Number of

Target Mutations

Amplicon

Locations in Exon(s)

NRAS 19 2, 3, 4
CTNNB1 5 3
PIK3CA 14 11, 22
FGFR3 9 7, 9, 14
KIT 8 11, 13, 17
EGFR 9 18, 19, 20, 21
BRAF 15 11, 15
RET 1 16
PTEN 3 5, 7
KRAS 22 2, 3, 4
TP53 7 4, 6, 7
Total 112  

 

 

Target Genes

Thyroid

Number of

Target Mutations

Amplicon

Locations in Exon(s)

NRAS 19 2, 3, 4
CTNNB1 5 3
PIK3CA 14 11, 22
FGFR3 9 7, 9, 14
cKIT - -
EGFR 10 18, 19, 20, 21
BRAF 15 11, 15
RET 1 16
PTEN 3 5, 7
KRAS 22 2, 3, 4
TP53 7 4, 6, 7
Total 105  

 

Target Genes

Melanoma

Number of

Target Mutations

Amplicon

Locations in Exon(s)

BRAF 37 15
NRAS 27 2, 3
CDKN2A 4 2
MAP2K1 4 3
FGFR3 9 7, 9, 14
AKT3 2 5, 6
cKIT 27 2, 11, 13, 17
PIK3CA 15 11
GNAQ 1 5
GNA11 1 5
Total 127  

 

FEATURES AND BENEFITS

  • Detect 5% variant frequency for mutation
  • Sample input: 10 µm section of FFPE sample with > 10 % tumor content
  • Get accurate interpretation and reporting with Vela Genomics Service and TheraKey Solution*
  • From sample to result in 2.5 days*
  • The OncoKey Core Primer Set can be used on different NGS instruments
  • 16 different barcoded DNA Adapters, each sufficient for 6 libraries
  • Combine NSCLC, CRC & Thyroid samples in one run
  • Seamless sample traceability and IT connectivity with Sentosa Link*
  • Flexible manual solution or automated workflow with 3h hands on time available
  • Automated workflow*

*Not available with/applicable for the Oncokey Core Primer Set (RUO)

 

WORKFLOW

SPECIFICATIONS (AUTOMATED WORKFLOW)

Attribute NSCLC CRC Thyroid Melanoma
Regulatory Status RUO
No. of Genes 11 11 10 10
No. of Amplicons 28 28 25 18
Target Mutations 113 112 105 127
Sample Input 10 µm section of FFPE sample with > 10% tumor content
Sample Throughput 7 samples and 1 system control sample
Median Coverage ≥ 1.000X 
Mutation Detection Sensitivity 5% - 10%
Hands on Time ≤ 3 hours
Turn around Time 2.5 days including overnight sample incubation
Pack Size 4x8

ORDERING INFORMATION

ProductPack SizeItem Number
Sentosa SQ 318 Chip Kit (RUO) 8 runs 300301
Sentosa SQ CRC Reagents (RUO) 4x8 300262
Sentosa SQ Colorectal Cancer Panel (RUO) 4x8 tests 690013
Sentosa SQ Melanoma Panel (RUO) 4x8 tests 690009
Sentosa SQ Melanoma Reagents (RUO) 4x8 300221
Sentosa SQ NSCLC Panel (RUO) 4x8 tests 690011
Sentosa SQ NSCLC Reagents (RUO) 4x8 300357
Sentosa SQ OncoKey Core Primer Set (RUO) 96 300694
Sentosa SQ Oncology Panel Solutions (RUO) 4x8 300224
Sentosa SQ Sequencing Reagents (RUO) 300310
Sentosa SQ Sequencing Solutions (RUO) 300314
Sentosa SQ Sequencing Supplies (RUO) 300304
Sentosa SQ Thyroid Cancer Panel (RUO) 4x8 tests 690035
Sentosa SQ Thyroid Cancer Reagents (RUO) 4x8 300382
Sentosa ST Template Beads (RUO) 300320
Sentosa ST Template Reagents (RUO) 300317
Sentosa ST Template Solutions (RUO) 300323
Sentosa ST Template Supplies (RUO) 300326
Sentosa SX FFPE II gDNA Kit (RUO) 4x8 tests 300231

For availability in your country, please contact your Vela Diagnostics sales representative. Availability is subject to country's regulatory approval.

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