Sentosa SQ Colorectal Cancer Panel

Detect sequence variants in colorectal cancer samples

DESCRIPTION

The Sentosa SQ CRC Panel is a Next-Generation Sequencing (NGS)-based test that simultaneously detects hot spot mutations in 11 genes from formalin-fixed paraffin-embedded (FFPE) samples for Research Use Only. This panel is intended to be used on the Sentosa SX101 with the Sentosa SX FFPE II gDNA Kit in conjunction with the Sentosa ST401 and Sentosa SQ301 instruments.

 

For Research Use Only. Not for use in diagnostics procedures.

TARGET GENES

Target GenesNumber of AmpliconsNumber of Target MutationsAmplicon Locations in Exon(s)
NRAS 3 19 2, 3, 4
CTNNB1 1 5 2
PIK3CA 2 14 11, 22
FGFR3 3 9 7, 9, 14
KIT 3 8 11, 13, 17
EGFR 4 9 18, 19, 20, 21
BRAF 2 15 11, 15
RET 1 1 16
PTEN 3 3 5, 7
KRAS 3 22 2, 3, 4
TP53 3 7 4, 6, 7
System Control 1 NA NA
Total 28 112  

FEATURES AND BENEFITS

Supporting Research

  • Minimal sample input -- 10 μm section of FFPE tissue or 5 ng DNA per library
  • Automated and user-friendly data analysis and results reporting with Sentosa SQ Reporter

Providing Laboratory Efficiency

  • From sample to result in about two days
  • Detection of up to 5% variant frequency
  • Seamless sample traceability and network connectivity

WORKFLOW

ORDERING INFORMATION

ProductPack SizeItem Number
Sentosa SQ 318 Chip Kit (8) RUO 8 runs 300301
Sentosa SQ CRC Panel Reagents (4x8) RUO 4x8 300262
Sentosa SQ Oncology Solutions (4x8) RUO 4x8 300224
Sentosa SQ Sequencing Reagents (8) RUO 8 300310
Sentosa SQ Sequencing Solutions (8) RUO 8 300314
Sentosa SQ Sequencing Supplies (8) RUO 8 300304
Sentosa ST Template Beads (8) RUO 8 300320
Sentosa ST Template Reagents (8) RUO 8 300317
Sentosa ST Template Solutions (8) RUO 8 300323
Sentosa ST Template Supplies (8) RUO 8 300326