Sentosa SQ Colorectal Cancer Panel

Detect sequence variants in colorectal cancer samples

DESCRIPTION

The Sentosa SQ CRC Panel is a Next-Generation Sequencing (NGS)-based test that simultaneously detects hot spot mutations in 11 genes from formalin-fixed paraffin-embedded (FFPE) samples for Research Use Only. This panel is intended to be used on the Sentosa SX101 with the Sentosa SX FFPE II gDNA Kit in conjunction with the Sentosa ST401 and Sentosa SQ301 instruments.

 

For Research Use Only. Not for use in diagnostics procedures.

TARGET GENES

Target GenesNumber of AmpliconsNumber of Target MutationsAmplicon Locations in Exon(s)
NRAS 3 19 2, 3, 4
CTNNB1 1 5 2
PIK3CA 2 14 11, 22
FGFR3 3 9 7, 9, 14
KIT 3 8 11, 13, 17
EGFR 4 9 18, 19, 20, 21
BRAF 2 15 11, 15
RET 1 1 16
PTEN 3 3 5, 7
KRAS 3 22 2, 3, 4
TP53 3 7 4, 6, 7
System Control 1 NA NA
Total 28 112  

FEATURES AND BENEFITS

Supporting Research

  • Minimal sample input -- 10 μm section of FFPE tissue or 5 ng DNA per library
  • Automated and user-friendly data analysis and results reporting with Sentosa SQ Reporter

Providing Laboratory Efficiency

  • From sample to result in about two days
  • Detection of up to 5% variant frequency
  • Seamless sample traceability and network connectivitiy with Sentosa link

WORKFLOW

ORDERING INFORMATION

ProductPack SizeItem Number
Sentosa SQ Colorectal Cancer Panel 4x8 tests 690013
Sentosa SX FFPE II gDNA Kit 4x8 tests 300231
Sentosa SQ 318 Chip Kit 8 runs 300301
Sentosa SQ Sequencing Kit 8 runs 690005
Sentosa ST Template Kit 8 runs 690007
Sentosa SX101 1 400089
Sentosa ST401 1 690027
Sentosa SQ301 (120 VAC) 1 690026